In anti-double-stranded DNA test, anti-double-stranded DNA present in the blood is checked. It is a special type of antinucleic antibody (ANA), which is found mainly in patients with autoimmune diseases such as: – Systemic lupus. It is in less than 1% of the body of healthy people.
To check the anti-double-stranded DNA antibody, the following test is used-
- ELISA( एंजाइम-लिंक्ड इम्मुनोसॉरबेंट ऎसे)
- Crithidia luciliae Immunofluorescence Test (क्रिथिडीया लुसीलिए इम्म्यूनोफ्लुओरेसेन्स टेस्ट )
- Radioimmunoassay(रेडियोइम्मुनोऎसे )
In many laboratory, anti-dsDNA enzyme-linked immunosorbent (ELISA) is used. This is a more reliable and sensitive test.
Occasionally if someone has a disease like Chronic Hepatitis, Primary Biliary Cirrhosis, and Infectious Mononucleosis, or if he is taking medicines such as Procainamide and Hydralazine, they may also have anti- Double stranded DNA is found.
In addition to anti-double stranded DNA, anti-single stranded DNA test is also done. But this auto-antibody is rarely checked and there is no relation with Lupus. But for other autoimmune diseases, it is checked.
ANA is a group of antinucleic antibodies. So if ANA test comes negative then you do not need to have anti-double-stranded DNA or any other tests related to it.